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FNDC5 Recombinant antibody

FNDC5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3

Cat no : 82671-1-RR

Synonyms

FNDC5, FRCP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FNDC5 Recombinant antibody (82671-1-RR)

    WB analysis using 82671-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82671-1-RR (FNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FNDC5 Recombinant antibody (82671-1-RR)

    IHC staining of mouse skeletal muscle using 82671-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FNDC5 Recombinant antibody (82671-1-RR)

    IHC staining of mouse skeletal muscle using 82671-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, mouse stomach tissue, rat stomach tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82671-1-RR targets FNDC5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FNDC5 fusion protein Ag29714
Full Name fibronectin type III domain containing 5
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC062297
Gene Symbol FNDC5
Gene ID (NCBI) 252995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

fibronectin type III domain containing 5(FNDC5) encodes 23 kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.

Protocols

Product Specific Protocols
WB protocol for FNDC5 antibody 82671-1-RRDownload protocol
IHC protocol for FNDC5 antibody 82671-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols