FNDC5 Recombinant antibody

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  WB analysis using 82671-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82671-1-RR (FNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 82671-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 82671-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FNDC5 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 82671-1-RR

Clone No.2H3

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

FNDC5, FRCP2

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Tested Applications

• Positive WB detected in: HepG2 cells, C2C12 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, mouse stomach tissue, rat stomach tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82671-1-RR targets FNDC5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FNDC5 fusion protein Ag29714
• Full Name: fibronectin type III domain containing 5
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC062297
• Gene Symbol: FNDC5
• Gene ID (NCBI): 252995
• RRID: AB_3086499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NAU1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

fibronectin type III domain containing 5(FNDC5) encodes 23 kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.