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CoraLite® Plus 488-conjugated FMR1 Monoclonal antibody
FMR1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B5B5
Cat no : CL488-66548
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | Jurkat cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66548 targets FMR1 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | FMR1 fusion protein Ag4697 |
Full Name | fragile X mental retardation 1 |
Calculated Molecular Weight | 632 aa, 71 kDa |
Observed Molecular Weight | 69-72 kDa |
GenBank Accession Number | BC038998 |
Gene Symbol | FMR1 |
Gene ID (NCBI) | 2332 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 FMR1 antibody CL488-66548 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |