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FMR1 Monoclonal antibody
FMR1 Monoclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), IP, ELISA, IF
Conjugate
Unconjugated
CloneNo.
2B5B5
Cat no : 66548-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSCT6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue, human liver tissue, human colon cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
66548-1-Ig targets FMR1 in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FMR1 fusion protein Ag4697 |
| Full Name | fragile X mental retardation 1 |
| Calculated Molecular Weight | 632 aa, 71 kDa |
| Observed Molecular Weight | 69-72 kDa |
| GenBank Accession Number | BC038998 |
| Gene Symbol | FMR1 |
| Gene ID (NCBI) | 2332 |
| RRID | AB_2881910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| IHC protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| IP protocol for FMR1 antibody 66548-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife FMRP promotes RNA localization to neuronal projections through interactions between its RGG domain and G-quadruplex RNA sequences.
| ||
Environ Res Polychlorinated biphenyls alter hepatic m6A mRNA methylation in a mouse model of environmental liver disease | ||
Front Mol Neurosci Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lisa (Verified Customer) (10-19-2021) | The antibody works quite well in WB, even if it is not possible to reuse the same diluted aliquot more than twice. I was not really satisfied of the results in IF, as the signal had a very high background.
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