FKBP2 Monoclonal antibody, PBS Only

FKBP2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7E8

Cat no : 66091-1-PBS

Synonyms

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8

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Validation Data Gallery

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  WB analysis of human cerebellum using 66091-1-Ig (same clone as 66091-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66091-1-Ig (same clone as 66091-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66091-1-Ig (same clone as 66091-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66091-1-Ig (same clone as 66091-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66091-1-Ig (same clone as 66091-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug FKBP2 Monoclonal antibody (66091-1-Ig, Clone:2H7E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

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Product Information

66091-1-PBS targets FKBP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBP2 fusion protein Ag19113
• Full Name: FK506 binding protein 2, 13kDa
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC003384
• Gene Symbol: FKBP2
• Gene ID (NCBI): 2286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).