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FKBP2 Monoclonal antibody

FKBP2 Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2H7E8

Cat no : 66091-1-Ig

Synonyms

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    WB analysis of human cerebellum using 66091-1-Ig

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    WB analysis of human brain using 66091-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    WB analysis of rat brain using 66091-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    WB analysis of pig liver using 66091-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    IHC staining of human prostate cancer using 66091-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FKBP2 Monoclonal antibody (66091-1-Ig)

    IHC staining of human prostate cancer using 66091-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using FKBP2 Monoclonal antibody (66091-1-Ig)

    FC experiment of HepG2 using 66091-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug FKBP2 Monoclonal antibody (66091-1-Ig, Clone:2H7E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHuman cerebellum tissue, human brain tissue, pig liver tissue, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66091-1-Ig targets FKBP2 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP2 fusion protein Ag19113
Full Name FK506 binding protein 2, 13kDa
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC003384
Gene Symbol FKBP2
Gene ID (NCBI) 2286
RRIDAB_11232416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Protocols

Product Specific Protocols
WB protocol for FKBP2 antibody 66091-1-IgDownload protocol
IHC protocol for FKBP2 antibody 66091-1-IgDownload protocol
FC protocol for FKBP2 antibody 66091-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Emerg Microbes Infect

Pharmacological suppression of glycogen synthase kinase-3 reactivates HIV-1 from latency via activating Wnt/β-catenin/TCF1 axis in CD4+ T cells.

Authors - Jing Wen
View Article