• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ChromoTek FITC Plus-conjugated FLT3 VHH

FITC-flt targets FLT3 in FC and IF applications and shows reactivity with Human samples

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

FITC Plus Fluorescent dye

Cat no : FITC-flt

Synonyms

FLT3-Nanobody, FLT3-Single domain antibody (sdAb), Receptor-type tyrosine-protein kinase, FL cytokine receptor, CD135, FLK2, FLK-2 STK1, STK-1, FLT3, FLT-3

Validation Data Gallery

  • 1X10^6 HEK FLT3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg FITC Plus conjugated-FLT3 VHH (FITC-flt). Cells were not fixed.

    1X10^6 HEK FLT3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg FITC Plus conjugated-FLT3 VHH (FITC-flt). Cells were not fixed.

  • HeLa cells transfected with human FLT-3 were immunostained with FITC Plus conjugated FLT3 VHH (1:500, green). Nuclei were stained with DAPI (blue or cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with FITC Plus conjugated FLT3 VHH can be performed live or after formaldehyde fixation.

    HeLa cells transfected with human FLT-3 were immunostained with FITC Plus conjugated FLT3 VHH (1:500, green). Nuclei were stained with DAPI (blue or cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with FITC Plus conjugated FLT3 VHH can be performed live or after formaldehyde fixation.

  • 0.5x10^6 KG-1 cells were surface stained with 2ug FITC Plus conjugated FLT3 VHH (FITC-flt3) (red) or unstained (blue).

    0.5x10^6 KG-1 cells were surface stained with 2ug FITC Plus conjugated FLT3 VHH (FITC-flt3) (red) or unstained (blue).

Product Information

FITC-flt targets FLT3 in FC and IF applications and shows reactivity with Human samples

ApplicationsFC, IF
ReactivityHuman
ConjugateFITC Plus Fluorescent dye
TypeNanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.433 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
RRIDAB_3101945
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundFLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.