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ChromoTek CoraLite® Plus 555-conjugated FLT3 VHH

CL555-flt targets FLT3 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-flt

Synonyms

FLT3-Nanobody, FLT3-Single domain antibody (sdAb), Receptor-type tyrosine-protein kinase, FL cytokine receptor, CD135, FLK2, FLK-2 STK1, STK-1, FLT3, FLT-3

Validation Data Gallery

  • 1X10^6 HEK FLT3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated FLT3 VHH (CL555-flt). Cells were not fixed.

    1X10^6 HEK FLT3 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated FLT3 VHH (CL555-flt). Cells were not fixed.

  • 0.5x10^6 KG-1 cells were surface stained with 1ug CoraLite® Plus 555 conjugated FLT3 VHH (CL555-flt3) (red) or unstained (blue).

    0.5x10^6 KG-1 cells were surface stained with 1ug CoraLite® Plus 555 conjugated FLT3 VHH (CL555-flt3) (red) or unstained (blue).

  • HeLa cells transfected with human FLT-3 were immunostained with CoraLite Plus 555 conjugated FLT3 VHH (1:500, red). Nuclei were stained with DAPI (blue or cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with CoraLite Plus 555 conjugated FLT3 VHH can be performed live or after formaldehyde fixation.

    HeLa cells transfected with human FLT-3 were immunostained with CoraLite Plus 555 conjugated FLT3 VHH (1:500, red). Nuclei were stained with DAPI (blue or cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with CoraLite Plus 555 conjugated FLT3 VHH can be performed live or after formaldehyde fixation.

Product Information

CL555-flt targets FLT3 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 555 Fluorescent Dye
TypeNanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.433 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
RRIDAB_3101946
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundFLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.