FITC Plus Mouse IgG2a Isotype Control (C1.18.4)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

C1.18.4

Cat no : FITC-65208

Synonyms

FITC Mouse IgG2a Isotype Control, IgG2a, IgG2a Isotype Control

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using FITC-65208

  1X10^6 human PBMCs were surface stained with 0.2 ug FITC Plus Anti-Mouse IgG2a Isotype Control (FITC-65208, Clone: C1.18.4) (red), or FITC Plus Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of mouse splenocytes using FITC-65208

  1X10^6 mouse splenocytes were surface stained with 0.2 ug FITC Anti-Mouse IgG2a Isotype Control (FITC-65208, Clone:C1.18.4) (red) or 0.2 ug FITC Anti-Mouse CD45.2 (FITC-65072) (green), or unstained (blue). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human PBMCs, mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_2883822
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.