CoraLite® Plus 488-conjugated FIS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FIS1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10956

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-10956

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FIS1 antibody (CL488-10956) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10956 targets FIS1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG1
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIS1 fusion protein Ag1409
• Full Name: fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC009428
• Gene Symbol: FIS1
• Gene ID (NCBI): 51024
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)