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FIS1 Polyclonal antibody
FIS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (6)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
308
Cat no : 10956-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, mouse heart tissue, SH-SY5Y cells, pig brain tissue, HeLa cells, rat heart tissue, Jurkat cells, mouse spleen tissue, rat spleen tissue, HepG2 cells, PC-12 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | rat brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, Hepa1-6 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:14000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 10 publications below |
WB | See 286 publications below |
IHC | See 8 publications below |
IF | See 35 publications below |
IP | See 4 publications below |
CoIP | See 1 publications below |
Product Information
10956-1-AP targets FIS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine, hamster, goat, squirrel |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FIS1 fusion protein Ag1409 |
Full Name | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC009428 |
Gene Symbol | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_2102532 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)
Protocols
Product Specific Protocols | |
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WB protocol for FIS1 antibody 10956-1-AP | Download protocol |
IHC protocol for FIS1 antibody 10956-1-AP | Download protocol |
IF protocol for FIS1 antibody 10956-1-AP | Download protocol |
IP protocol for FIS1 antibody 10956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Distinct fission signatures predict mitochondrial degradation or biogenesis.
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Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.
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Cell Stem Cell AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells.
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Nat Metab Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Megan (Verified Customer) (06-22-2021) | RPE cell over-expressing pcDNA hFis1. Note 1:500 was used here to detect over-expressed Fis1, but we also use 1:100 when detecting endogenous Fis1. Also successfully used to detect endogenous Fis1 levels via Western Blot (used @ 1:1000).
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FH Siting (Verified Customer) (01-28-2020) | This antibody worked perfectly for western blot.
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FH Chun (Verified Customer) (07-03-2019) | This antibody is excellent/
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FH Kishor (Verified Customer) (12-13-2018) | I got good results in both rat liver protein and H69 cells.
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