FIP1L1 Recombinant antibody, PBS Only

FIP1L1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240658C4

Cat no : 83863-2-PBS

Synonyms

RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1, 240658C4

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Validation Data Gallery

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  WB analysis using 83863-2-RR (same clone as 83863-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83863-2-RR (same clone as 83863-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 83863-2-RR (same clone as 83863-2-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83863-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

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Product Information

The immunogen of 83863-2-PBS is FIP1L1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FIP1L1 fusion protein Ag2921
• Full Name: FIP1 like 1 (S. cerevisiae)
• Calculated Molecular Weight: 594 aa, 66 kDa
• Observed Molecular Weight: 67-80 kDa
• GenBank Accession Number: BC017724
• Gene Symbol: FIP1L1
• Gene ID (NCBI): 81608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).