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FIP1L1 Recombinant antibody

FIP1L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240658C4

Cat no : 83863-2-RR

Synonyms

RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1, 240658C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIP1L1 Recombinant antibody (83863-2-RR)

    WB analysis using 83863-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using FIP1L1 Recombinant antibody (83863-2-RR)

    FC experiment of A549 using 83863-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using FIP1L1 Recombinant antibody (83863-2-RR)

    FC experiment of U-2 OS using 83863-2-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83863-2-RR

    Affinity and Kinetic Characterization of 83863-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

Tested Applications

Positive WB detected inHeLa cells, U-251 cells, HEK-293T cells, MCF-7 cells, mouse thymus tissue
Positive FC (Intra) detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83863-2-RR is FIP1L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIP1L1 fusion protein Ag2921
Full Name FIP1 like 1 (S. cerevisiae)
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 67-80 kDa
GenBank Accession NumberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).

Protocols

Product Specific Protocols
WB protocol for FIP1L1 antibody 83863-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols