CoraLite®594-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A9E8

Cat no : CL594-66985

Synonyms

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66985

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 FBL antibody (CL594-66985, Clone: 3A9E8 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66985

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human FBL (CL594-66985, Clone:3A9E8)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66985 targets FBL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FBL fusion protein Ag8841
• Full Name: fibrillarin
• Calculated Molecular Weight: 321 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019260
• Gene Symbol: FBL
• Gene ID (NCBI): 2091
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).