FANCM Recombinant antibody

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  IP experiment of HepG2 using 82897-2-RR

  IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug.

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  IHC staining of mouse testis using 82897-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82897-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82897-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of PC-3 using 82897-2-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

FANCM Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 82897-2-RR

Clone No.230233G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

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Tested Applications

• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82897-2-RR targets FANCM in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FANCM fusion protein Ag34447
• Full Name: Fanconi anemia, complementation group M
• GenBank Accession Number: BC140776
• Gene Symbol: FANCM
• Gene ID (NCBI): 57697
• RRID: AB_3670625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IYD8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.