FANCM Recombinant antibody, PBS Only

FANCM Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230233G5

Cat no : 82897-2-PBS

Synonyms

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

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Validation Data Gallery

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  IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)

  IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug. This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

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Product Information

82897-2-PBS targets FANCM in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FANCM fusion protein Ag34447
• Full Name: Fanconi anemia, complementation group M
• GenBank Accession Number: BC140776
• Gene Symbol: FANCM
• Gene ID (NCBI): 57697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.