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CoraLite®594-conjugated Emerin Polyclonal antibody

Emerin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10351

Synonyms

EDMD, EMD, emerin, LEMD5, STA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Emerin Polyclonal antibody (CL594-10351)

    IF Staining of HeLa using CL594-10351

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Emerin antibody (CL594-10351) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Emerin Polyclonal antibody (CL594-10351)

    FC experiment of HeLa using CL594-10351

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Emerin (CL594-10351) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10351 targets Emerin in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Emerin fusion protein Ag0236
Full Name emerin
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
RRIDAB_2919729
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Protocols

Product Specific Protocols
IF protocol for CL594 Emerin antibody CL594-10351Download protocol
FC protocol for CL594 Emerin antibody CL594-10351Download protocol
Standard Protocols
Click here to view our Standard Protocols