ESYT1 Monoclonal antibody, PBS Only

ESYT1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C12E4

Cat no : 67688-1-PBS

Synonyms

E Syt1, ESYT1, E-Syt1, Extended synaptotagmin 1, Extended synaptotagmin-1, FAM62A, KIAA0747, MBC2

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Validation Data Gallery

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  WB analysis using 67688-1-Ig (same clone as 67688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (ESYT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

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  WB analysis using 67688-1-Ig (same clone as 67688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67688-1-Ig (same clone as 67688-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67688-1-Ig (same clone as 67688-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ESYT1 antibody (67688-1-Ig, Clone: 2C12E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67688-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67688-1-PBS targets ESYT1 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ESYT1 fusion protein Ag16840
• Full Name: family with sequence similarity 62 (C2 domain containing), member A
• Calculated Molecular Weight: 1104 aa, 123 kDa
• Observed Molecular Weight: 123 kDa
• GenBank Accession Number: BC004998
• Gene Symbol: FAM62A
• Gene ID (NCBI): 23344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.