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  • Featured Product
  • KD/KO Validated

ESYT1 Monoclonal antibody

ESYT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C12E4

Cat no : 67688-1-Ig

Synonyms

E Syt1, ESYT1, E-Syt1, Extended synaptotagmin 1, Extended synaptotagmin-1, FAM62A, KIAA0747, MBC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis using 67688-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (ESYT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis using 67688-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ESYT1 Monoclonal antibody (67688-1-Ig)

    WB analysis of HSC-T6 using 67688-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67688-1-Ig (FAM62A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ESYT1 Monoclonal antibody (67688-1-Ig)

    IF Staining of U2OS using 67688-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ESYT1 antibody (67688-1-Ig, Clone: 2C12E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HSC-T6 cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67688-1-Ig targets ESYT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ESYT1 fusion protein Ag16840
Full Name family with sequence similarity 62 (C2 domain containing), member A
Calculated Molecular Weight 1104 aa, 123 kDa
Observed Molecular Weight123 kDa
GenBank Accession NumberBC004998
Gene Symbol ESYT1
Gene ID (NCBI) 23344
RRIDAB_2882881
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ESYT1 antibody 67688-1-IgDownload protocol
IF protocol for ESYT1 antibody 67688-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair

Authors - Arsila P. K. Ashraf
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Shihab (Verified Customer) (09-17-2021)

This antibody was used in a western blot application to identify recombinant Esyt1 prescence. Using 1:500 at the first attempt, this antibody was able to successfully provide a band at the correct size. Furthermore, the band was clean which was an added bonus. I will be reducing the concentration of antibody used and also trying this antibody in other applications as well as primary cell lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 (will be reducing)
  • Cell Tissue Type: HEK293 transfected with Esyt1