ESRRB Monoclonal antibody, PBS Only

ESRRB Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E10C1

Cat no : 66818-1-PBS

Synonyms

DFNB35, ERR2, ERRb, ERRbeta, ERRbeta 2, ESRL2, ESRRB, ESRRB protein, estrogen related receptor beta, NR3B2

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Validation Data Gallery

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  WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66818-1-PBS targets ESRRB in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ESRRB fusion protein Ag18626
• Full Name: estrogen-related receptor beta
• Calculated Molecular Weight: 508 aa, 56 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC131517
• Gene Symbol: ESRRB
• Gene ID (NCBI): 2103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.