CoraLite® Plus 488-conjugated ERp72 Polyclonal antibody

• Featured Product
• KD/KO Validated

ERp72 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14712

Synonyms

ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4

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Validation Data Gallery

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  IF Staining of PC-3 using CL488-14712

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 488 ERp72 antibody (CL488-14712) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14712 targets ERp72 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERp72 fusion protein Ag6431
• Full Name: protein disulfide isomerase family A, member 4
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: BC000425
• Gene Symbol: ERp72
• Gene ID (NCBI): 9601
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.