FITC Plus-conjugated EPHX2 Monoclonal antibody

• 

  IF Staining of A549 using FITC-67322

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FITC Plus EPHX2 antibody (FITC-67322, Clone: 4H6C8 ) at dilution of 1:50.

• Featured Product
• KD/KO Validated

EPHX2 Monoclonal Antibody for IF/ICC

Cat No. FITC-67322

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IF/ICC

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-67322 targets EPHX2 in IF/ICC applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHX2 fusion protein Ag4870
• Full Name: epoxide hydrolase 2, cytoplasmic
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC013874
• Gene Symbol: EPHX2
• Gene ID (NCBI): 2053
• RRID: AB_3673875
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P34913
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).