EPHX2 Monoclonal antibody

• Featured Product
• KD/KO Validated

EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4H6C8

Cat no : 67322-1-Ig

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

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Validation Data Gallery

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  WB analysis using 67322-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67322-1-Ig

  WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

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  IHC staining of human breast cancer using 67322-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of human breast cancer using 67322-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens).

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  IF Staining of A549 using 67322-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using EPHX2 antibody (67322-1-Ig, Clone: 4H6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, A549 cells, Jurkat cells, pig liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67322-1-Ig targets EPHX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPHX2 fusion protein Ag4870
• Full Name: epoxide hydrolase 2, cytoplasmic
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 56-63 kDa
• GenBank Accession Number: BC013874
• Gene Symbol: EPHX2
• Gene ID (NCBI): 2053
• RRID: AB_2882582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).