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CoraLite®594-conjugated eIF4G2/DAP5 Monoclonal antibody

eIF4G2/DAP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C11A3

Cat no : CL594-67428

Synonyms

EIF4G2, Death associated protein 5, DAP-5, DAP5, DAP 5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated eIF4G2/DAP5 Monoclonal ant (CL594-67428)

    IF Staining of HepG2 using CL594-67428

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF4G2 antibody (CL594-67428, Clone: 3C11A3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated eIF4G2/DAP5 Monoclonal ant (CL594-67428)

    FC experiment of HeLa using CL594-67428

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human EIF4G2 (CL594-67428, Clone:3C11A3) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67428 targets eIF4G2/DAP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12080
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2920134
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 eIF4G2/DAP5 antibody CL594-67428Download protocol
FC protocol for CL594 eIF4G2/DAP5 antibody CL594-67428Download protocol
Standard Protocols
Click here to view our Standard Protocols