eIF4G2/DAP5 Monoclonal antibody

• Featured Product
• KD/KO Validated

eIF4G2/DAP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3C11A3

Cat no : 67428-1-Ig

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, FLJ41344, NAT1, p97

---

Validation Data Gallery

• 

  WB analysis using 67428-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (EIF4G2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67428-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67428-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67428-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using eIF4G2/DAP5 antibody (67428-1-Ig, Clone: 3C11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

67428-1-Ig targets eIF4G2/DAP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: eIF4G2/DAP5 fusion protein Ag12080
• Full Name: eukaryotic translation initiation factor 4 gamma, 2
• Calculated Molecular Weight: 907 aa, 102 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC043149
• Gene Symbol: EIF4G2
• Gene ID (NCBI): 1982
• RRID: AB_2882667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell; ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.; Authors - Yichen Xu ; KD Validated