EIF2AK2, PKR Monoclonal antibody, PBS Only

EIF2AK2, PKR Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8A4

Cat no : 66646-1-PBS

Synonyms

EIF2AK2, PKR, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66646-1-Ig (same clone as 66646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66646-1-Ig (same clone as 66646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

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Product Information

66646-1-PBS targets EIF2AK2, PKR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC093676
• Gene Symbol: EIF2AK2
• Gene ID (NCBI): 5610
• RRID: AB_2882004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.