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EIF2AK2 Monoclonal antibody

EIF2AK2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8A4

Cat no : 66646-1-Ig

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2AK2 Monoclonal antibody (66646-1-Ig)

    WB analysis using 66646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2 Monoclonal antibody (66646-1-Ig)

    IHC staining of human testis using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using EIF2AK2 Monoclonal antibody (66646-1-Ig)

    IHC staining of human testis using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2 Monoclonal antibody (66646-1-Ig)

    IHC staining of human kidney using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF2AK2 Monoclonal antibody (66646-1-Ig)

    IHC staining of human kidney using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66646-1-Ig targets EIF2AK2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2AK2 fusion protein Ag13010
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2882004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Protocols

Product Specific Protocols
WB protocol for EIF2AK2 antibody 66646-1-IgDownload protocol
IHC protocol for EIF2AK2 antibody 66646-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

The impact of single-stranded RNAs on the dimerization of double-stranded RNA-dependent protein kinase PKR

Authors - Tomoya Kitano
View Article