EIF2AK2, PKR Monoclonal antibody

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  WB analysis using 66646-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EIF2AK2, PKR Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66646-1-Ig

Clone No.2A8A4

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

EIF2AK2, PKR, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, EC:2.7.11.1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66646-1-Ig targets EIF2AK2, PKR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC093676
• Gene Symbol: EIF2AK2
• Gene ID (NCBI): 5610
• RRID: AB_2882004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P19525
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; The impact of single-stranded RNAs on the dimerization of double-stranded RNA-dependent protein kinase PKR; Authors - Tomoya Kitano