EIF2AK2, PKR Monoclonal antibody
EIF2AK2, PKR Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A8A4
Cat no : 66646-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
Positive IHC detected in | human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
66646-1-Ig targets EIF2AK2, PKR in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EIF2AK2, PKR fusion protein Ag13010 |
Full Name | eukaryotic translation initiation factor 2-alpha kinase 2 |
Calculated Molecular Weight | 551 aa, 62 kDa |
Observed Molecular Weight | 65-68 kDa |
GenBank Accession Number | BC093676 |
Gene Symbol | EIF2AK2 |
Gene ID (NCBI) | 5610 |
RRID | AB_2882004 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.
Protocols
Product Specific Protocols | |
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WB protocol for EIF2AK2, PKR antibody 66646-1-Ig | Download protocol |
IHC protocol for EIF2AK2, PKR antibody 66646-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Biophys Res Commun The impact of single-stranded RNAs on the dimerization of double-stranded RNA-dependent protein kinase PKR |