EFEMP2 Polyclonal antibody, PBS Only

EFEMP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12004-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12004-1-AP (same clone as 12004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12004-1-AP (same clone as 12004-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

  IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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Product Information

12004-1-PBS targets EFEMP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFEMP2 fusion protein Ag2640
• Full Name: EGF-containing fibulin-like extracellular matrix protein 2
• Calculated Molecular Weight: 443 aa, 49 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC010456
• Gene Symbol: EFEMP2
• Gene ID (NCBI): 30008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.