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EFEMP2 Polyclonal antibody

EFEMP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12004-1-AP

Synonyms

Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis using 12004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis of mouse kidney using 12004-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis of K-562 using 12004-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IP experiment of mouse kidney using 12004-1-AP

    IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human prostate cancer using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human prostate cancer using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human prostate cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFEMP2 fusion protein Ag2640
Full Name EGF-containing fibulin-like extracellular matrix protein 2
Calculated Molecular Weight 443 aa, 49 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC010456
Gene Symbol EFEMP2
Gene ID (NCBI) 30008
RRIDAB_10667002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Protocols

Product Specific Protocols
WB protocol for EFEMP2 antibody 12004-1-APDownload protocol
IHC protocol for EFEMP2 antibody 12004-1-APDownload protocol
IP protocol for EFEMP2 antibody 12004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
View Article
humanWB

Cell Mol Biol Lett

EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells

Authors - Xin Shen
View Article