EFEMP2 Polyclonal antibody

EFEMP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12004-1-AP

Synonyms

Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4

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Validation Data Gallery

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  WB analysis using 12004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12004-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12004-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12004-1-AP

  IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HT-29 cells, K-562 cells, mouse kidney tissue, JAR cells, SW480 cells, human heart tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human heart tissue, human prostate cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12004-1-AP targets EFEMP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFEMP2 fusion protein Ag2640
• Full Name: EGF-containing fibulin-like extracellular matrix protein 2
• Calculated Molecular Weight: 443 aa, 49 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC010456
• Gene Symbol: EFEMP2
• Gene ID (NCBI): 30008
• RRID: AB_10667002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Publications

Species	Application	Title
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang
human	WB	Cell Mol Biol Lett; EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells; Authors - Xin Shen