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CoraLite®555-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E4D5

Cat no : CL555-67550

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated EEF2 Monoclonal antibody (CL555-67550)

    IF Staining of HepG2 using CL555-67550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 EEF2 antibody (CL555-67550, Clone: 1E4D5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated EEF2 Monoclonal antibody (CL555-67550)

    FC experiment of HeLa using CL555-67550

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human EEF2 (CL555-67550, Clone:1E4D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67550 targets EEF2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13859
Full Name eukaryotic translation elongation factor 2
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2919703
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL555 EEF2 antibody CL555-67550Download protocol
FC protocol for CL555 EEF2 antibody CL555-67550Download protocol
Standard Protocols
Click here to view our Standard Protocols