CoraLite® Plus 488-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D5

Cat no : CL488-67550

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67550

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 EEF2 antibody (CL488-67550, Clone: 1E4D5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67550 targets EEF2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF2 fusion protein Ag13859
• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• RRID: AB_2919498
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.