EEF1B2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EEF1B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10095-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10095-2-AP (same clone as 10095-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

  WB result of EEF1B2 antibody (10095-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 10095-2-AP (same clone as 10095-2-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10095-2-AP (same clone as 10095-2-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of RAW264.7 using 10095-2-AP (same clone as 10095-2-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10095-2-AP (same clone as 10095-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 10095-2-AP (same clone as 10095-2-PBS)

  IP result of anti-EEF1B2 (IP:10095-2-AP, 4ug; Detection:10095-2-AP 1:6000) with Jurkat cells lysate 1400 ug. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10095-2-AP (same clone as 10095-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (10095-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

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Product Information

10095-2-PBS targets EEF1B2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1B2 fusion protein Ag0135
• Full Name: eukaryotic translation elongation factor 1 beta 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC000211
• Gene Symbol: EEF1B2
• Gene ID (NCBI): 1933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.