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EEF1B2 Polyclonal antibody

Name: EEF1B2 Polyclonal antibody
Brand: Proteintech
SKU: 10095-2-AP

EEF1B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10095-2-AP

Synonyms

Elongation factor 1 beta, EF-1-beta, EF1B, EF 1 beta, EEF1B1

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 10095-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10095-2-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10095-2-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10095-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10095-2-AP
WB result of EEF1B2 antibody (10095-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.
WB analysis of RAW264.7 using 10095-2-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10095-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 10095-2-AP
IP result of anti-EEF1B2 (IP:10095-2-AP, 4ug; Detection:10095-2-AP 1:6000) with Jurkat cells lysate 1400 ug.
IHC staining of human pancreas cancer using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10095-2-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10095-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (10095-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 10095-2-AP

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Tested Applications

Positive WB detected in	PC-3 cells, RAW264.7, Jurkat cells, HEK-293 cells, HeLa cells, SKOV-3 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human colon tissue, human breast cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

10095-2-AP targets EEF1B2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EEF1B2 fusion protein Ag0135
Full Name	eukaryotic translation elongation factor 1 beta 2
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	30-34 kDa
GenBank Accession Number	BC000211
Gene Symbol	EEF1B2
Gene ID (NCBI)	1933
RRID	AB_2096987
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.

Protocols

Product Specific Protocols
WB protocol for EEF1B2 antibody 10095-2-AP	Download protocol
IHC protocol for EEF1B2 antibody 10095-2-AP	Download protocol
IF protocol for EEF1B2 antibody 10095-2-AP	Download protocol
IP protocol for EEF1B2 antibody 10095-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	RIP	Nat Cell Biol The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1. Authors - Ji-Hang Yuan View Article
human	WB,IF,IP	Mol Cell Biol Translational repression by RNA-binding protein TIAR. Authors - Mazan-Mamczarz Krystyna K View Article
human	WB	Front Microbiol Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins. Authors - Shuhei Sammaibashi View Article
mouse, human	WB, IHC, IF	PLoS One Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2. Authors - Yuan Cao KD Validated View Article
human	WB	Biotechniques En masse analysis of nascent translation using microarrays. Authors - Mazan-Mamczarz Krystyna K View Article

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10095-2-AP

WB analysis of PC-3 using 10095-2-AP

WB analysis of SKOV-3 using 10095-2-AP

WB analysis of HeLa using 10095-2-AP

WB analysis of HeLa using 10095-2-AP

WB analysis of RAW264.7 using 10095-2-AP

WB analysis of Jurkat using 10095-2-AP

IP experiment of Jurkat using 10095-2-AP

IHC staining of human pancreas cancer using 10095-2-AP

IHC staining of human pancreas cancer using 10095-2-AP

IHC staining of human breast cancer using 10095-2-AP

IHC staining of human breast cancer using 10095-2-AP

IHC staining of human colon using 10095-2-AP

IHC staining of human colon using 10095-2-AP

IF Staining of MCF-7 using 10095-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The MBNL3 splicing factor promotes hepatocellular carcinoma by increasing PXN expression through the alternative splicing of lncRNA-PXN-AS1.

Translational repression by RNA-binding protein TIAR.

Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.

Characterisation of Translation Elongation Factor eEF1B Subunit Expression in Mammalian Cells and Tissues and Co-Localisation with eEF1A2.

En masse analysis of nascent translation using microarrays.