CoraLite® Plus 647-conjugated EED Monoclonal antibody

EED Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D11D4

Cat no : CL647-67756

Synonyms

WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development

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Validation Data Gallery

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  IF Staining of mouse testis using CL647-67756

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

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  IF Staining of mouse testis using CL647-67756

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-67756

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated EED Monoclonal antibody (CL647-67756, Clone:1D11D4)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b isotype control (11B8C4) (CL647-66360-3, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67756 targets EED in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EED fusion protein Ag10369
• Full Name: embryonic ectoderm development
• Calculated Molecular Weight: 466 aa, 53 kDa
• GenBank Accession Number: BC068995
• Gene Symbol: EED
• Gene ID (NCBI): 8726
• RRID: AB_2920305
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011