CoraLite® Plus 488-conjugated E2F7 Recombinant antibody

E2F7 Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230073C2

Cat no : CL488-82887

Synonyms

E2F transcription factor 7, E2F7, FLJ12981

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Validation Data Gallery

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  IF Staining of HeLa using CL488-82887

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 E2F7 antibody (CL488-82887, Clone: 230073C2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82887 targets E2F7 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: E2F7 fusion protein Ag21599
• Full Name: E2F transcription factor 7
• Calculated Molecular Weight: 911 aa, 100 kDa
• GenBank Accession Number: BC136366
• Gene Symbol: E2F7
• Gene ID (NCBI): 144455
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )