CoraLite®594-conjugated Desmin Monoclonal antibody

Desmin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B1A11

Cat no : CL594-67793

Synonyms

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

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Validation Data Gallery

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  IF Staining of human colon using CL594-67793

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 Desmin antibody (CL594-67793, Clone: 2B1A11 ) at dilution of 1:200. DAPI (blue).

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Tested Applications

• Positive IF-P detected in: human colon tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67793 targets Desmin in IF-P applications and shows reactivity with Human, pig, mouse samples.

• Tested Reactivity: Human, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Desmin fusion protein Ag9675
• Full Name: desmin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC032116
• Gene Symbol: Desmin
• Gene ID (NCBI): 1674
• RRID: AB_2920184
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.