Desmin Monoclonal antibody

Desmin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B1A11

Cat no : 67793-1-Ig

Synonyms

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793



Tested Applications

Positive WB detected inpig heart tissue, mouse skeletal muscle tissue, mouse testis tissue, pig skeletal muscle, pig colon
Positive IHC detected inhuman colon tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon tissue, human appendicitis tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

67793-1-Ig targets Desmin in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Desmin fusion protein Ag9675
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 67793-1-IgDownload protocol
IHC protocol for Desmin antibody 67793-1-IgDownload protocol
IF protocol for Desmin antibody 67793-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Exp Cell Res

Bone marrow-derived mesenchymal stem cells transplantation attenuates renal fibrosis following acute kidney injury by repairing the peritubular capillaries

Authors - Weifeng Hu
mouseIF

DNA Cell Biol

Single-Cell RNA Sequencing Technology Revealed the Pivotal Role of Fibroblast Heterogeneity in Angiotensin II-Induced Abdominal Aortic Aneurysms.

Authors - Yingzheng Weng