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CoraLite®594-conjugated Desmin Polyclonal antibody

Desmin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16520

Synonyms

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite®594-conjugated Desmin Polyclonal antibody (CL594-16520)

    FC experiment of C2C12 using CL594-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Desmin (CL594-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-16520 targets Desmin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Desmin fusion protein Ag9742
Full Name desmin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 52-53 kDa
GenBank Accession NumberBC032116
Gene Symbol DES
Gene ID (NCBI) 1674
RRIDAB_2919835
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
IF protocol for CL594 Desmin antibody CL594-16520Download protocol
Standard Protocols
Click here to view our Standard Protocols