Filter: AllIF-PIF/ICCIFFC (Intra)

IF Staining of rat heart using CL488-16520
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:100.
IF Staining of mouse heart using CL488-16520
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.
IF Staining of mouse heart using CL488-16520
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.
IF Staining of mouse heart using CL488-16520
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488-conjugated Desmin antibody (CL488-16520) at dilution of 1:100.
IF Staining of C2C12 using CL488-16520
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.
IF Staining of mouse small intestine using CL488-16520
Adult mouse small intestine was co-stained with desmin (CL488-16520, in cyan), E-cadherin (20874-1-AP, in red), and PCNA (60097-1-Ig, in green). Desmin marks the stromal cells and smooth muscle, E-cadherin marks the gut epithelial cells, and PCNA marks proliferating cells. The image was created in paid partnership with @Immunofluorescence on Instagram.
IF Staining of mouse heart using CL488-16520
E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.
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E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.
FC experiment of C2C12 using CL488-16520
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmin (CL488-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CoraLite® Plus 488-conjugated Desmin Polyclonal antibody

Name: CoraLite® Plus 488-conjugated Desmin Polyclonal antibody
Brand: Proteintech
SKU: CL488-16520

Desmin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL488-16520

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

DES

Formulation: PBS, Proclin 300, BSA, Glycerol

PBS, Proclin 300, BSA, Glycerol

Conjugate: CoraLite® Plus 488

Unconjugated CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IF-P detected in	mouse heart tissue, mouse small intestine tissue, rat heart tissue
Positive IF/ICC detected in	C2C12 cells
Positive FC (Intra) detected in	C2C12 cells

Recommended dilution

Application	Dilution
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16520 targets Desmin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9742 Product name: Recombinant human Desmin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 121-470 aa of BC032116 Sequence: NYIEKVRFLEQQNAALAAEVNRLKGREPTRVAELYEEELRELRRQVEVLTNQRARVDVERDNLLDDLQRLKAKLQEEIQLKEEAENNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEASGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL Predict reactive species
Full Name	desmin
Calculated Molecular Weight	470 aa, 54 kDa
Observed Molecular Weight	52-53 kDa
GenBank Accession Number	BC032116
Gene Symbol	Desmin
Gene ID (NCBI)	1674
RRID	AB_2883084
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	493 nm / 522 nm
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17661
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.