CoraLite® Plus 488-conjugated Desmin Polyclonal antibody

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  IF Staining of rat heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:100.

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  IF Staining of mouse heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

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  IF Staining of mouse heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

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  IF Staining of mouse heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488-conjugated Desmin antibody (CL488-16520) at dilution of 1:100.

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  IF Staining of C2C12 using CL488-16520

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-16520

  Adult mouse small intestine was co-stained with desmin (CL488-16520, in cyan), E-cadherin (20874-1-AP, in red), and PCNA (60097-1-Ig, in green). Desmin marks the stromal cells and smooth muscle, E-cadherin marks the gut epithelial cells, and PCNA marks proliferating cells. The image was created in paid partnership with @Immunofluorescence on Instagram.

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  IF Staining of mouse heart using CL488-16520

  E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

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  FC experiment of C2C12 using CL488-16520

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmin (CL488-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Desmin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL488-16520

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

DES

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Tested Applications

• Positive IF-P detected in: mouse heart tissue, mouse small intestine tissue, rat heart tissue
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

CL488-16520 targets Desmin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Desmin fusion protein Ag9742
• Full Name: desmin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 52-53 kDa
• GenBank Accession Number: BC032116
• Gene Symbol: Desmin
• Gene ID (NCBI): 1674
• RRID: AB_2883084
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17661
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
mouse	FC	J Vasc Res; Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis; Authors - Wei Chang