CoraLite® Plus 647-conjugated Decorin Monoclonal antibody

Decorin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A4E4

Cat no : CL647-66847

Synonyms

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Validation Data Gallery

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  IF Staining of BxPC-3 using CL647-66847

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CoraLite® Plus 647 Decorin antibody (CL647-66847, Clone: 3A4E4 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66847 targets Decorin in IF/ICC applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Decorin fusion protein Ag6275
• Full Name: decorin
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 40 kDa, 100 kDa
• GenBank Accession Number: BC005322
• Gene Symbol: Decorin
• Gene ID (NCBI): 1634
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.