CoraLite® Plus 488-conjugated Decorin Monoclonal antibody

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  IF Staining of BxPC-3 using CL488-66847

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 Decorin antibody (CL488-66847, Clone: 3A4E4 ) at dilution of 1:200.

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  FC experiment of BxPC-3 using CL488-66847

  1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Decorin Monoclonal antibody (CL488-66847, Clone:3A4E4)(red), or 0.4 ug CoraLite® Plus 488-conjugated Mouse IgG1 isotype control Mouse McAb (CL488-66360, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Decorin Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66847

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  CoraLite® Plus 488

Unconjugated CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive IF/ICC detected in: BxPC-3 cells
• Positive FC (Intra) detected in: BxPC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66847 targets Decorin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Decorin fusion protein Ag6275
• Full Name: decorin
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 40 kDa, 100 kDa
• GenBank Accession Number: BC005322
• Gene Symbol: Decorin
• Gene ID (NCBI): 1634
• RRID: AB_3672925
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07585
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.