CoraLite® Plus 488-conjugated DUT Polyclonal antibody

DUT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13740

Synonyms

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

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Validation Data Gallery

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  IF Staining of HeLa using CL488-13740

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DUT antibody (CL488-13740) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of U2OS using CL488-13740

  1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DUT Polyclonal Antibody (CL488-13740)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13740 targets DUT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUT fusion protein Ag4677
• Full Name: deoxyuridine triphosphatase
• Calculated Molecular Weight: 252 aa, 27 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC033645
• Gene Symbol: DUT
• Gene ID (NCBI): 1854
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.