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CoraLite® Plus 488-conjugated DSE Monoclonal antibody

DSE Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C9F7

Cat no : CL488-60041

Synonyms

dermatan sulfate epimerase, DS epimerase, DSE, DSEPI, SART 2, SART2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DSE Monoclonal antib (CL488-60041)

    IF Staining of HeLa using CL488-60041

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DSE antibody (CL488-60041, Clone: 2C9F7 ) at dilution of 1:200, CL594-Phalloidin (red).

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60041 targets DSE in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen DSE fusion protein Ag0695
Full Name dermatan sulfate epimerase
Calculated Molecular Weight 958 aa, 110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC039245
Gene Symbol DSE
Gene ID (NCBI) 29940
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodEuglobulin precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DSE, also named as SART2 and DSEPI, is an enzyme that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour-associated antigen. DSE is recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. DSE is a potential candidate for a tumour antigen with immunogenicity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DSE antibody CL488-60041Download protocol
Standard Protocols
Click here to view our Standard Protocols