DPP10 Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 84127-2-RR (same clone as 84127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01035-3

  Sandwich ELISA standard curve of MP01035-3, Human DPP10 Recombinant Matched Antibody Pair - PBS only. 84127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3231. 84127-3-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

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  Sample test of MP01035-3

  HT-29 cell lysate was measured. The human DPP10 concentration of detected samples was determined to be 3,448.25 ng/mL (based on a 4.4 mg/mL extract load).

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  IP experiment of pig brain using 84127-2-RR (same clone as 84127-2-PBS)

  IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01035-1

  Cytometric bead array standard curve of MP01035-1, DPP10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84127-3-PBS. Detection antibody: 84127-2-PBS. Standard: Ag3231. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 84127-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

DPP10 {ptg:Brand} Recombinant Antibody for WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84127-2-PBS

Clone No.241446A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

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Product Information

84127-2-PBS targets DPP10 as part of a matched antibody pair:

MP01035-1: 84127-3-PBS capture and 84127-2-PBS detection (validated in Cytometric bead array)

MP01035-3: 84127-2-PBS capture and 84127-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DPP10 fusion protein Ag3231
• Full Name: dipeptidyl-peptidase 10
• Calculated Molecular Weight: 796 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC030832
• Gene Symbol: DPP10
• Gene ID (NCBI): 57628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N608
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.