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  • Western Blot (WB) analysis of various lysates using DPP10 Recombinant antibody (84127-2-RR)

    WB analysis using 84127-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of pig brain tissue using DPP10 Recombinant antibody (84127-2-RR)

    IP experiment of pig brain using 84127-2-RR

    IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug.

  • Affinity and Kinetic Characterization of 84127-2-RR

    Affinity and Kinetic Characterization of 84127-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

DPP10 Recombinant antibody

DPP10 {ptg:Brand} Recombinant Antibody for WB, IP, ELISA
Cat No. 84127-2-RR
Clone No.241446A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, ELISA

Synonyms

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inpig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84127-2-RR targets DPP10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DPP10 fusion protein Ag3231 Predict reactive species
Full Name dipeptidyl-peptidase 10
Calculated Molecular Weight 796 aa, 91 kDa
Observed Molecular Weight91 kDa
GenBank Accession NumberBC030832
Gene Symbol DPP10
Gene ID (NCBI) 57628
RRIDAB_3671686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N608
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.

Protocols

Product Specific Protocols
WB protocol for DPP10 antibody 84127-2-RRDownload protocol
IP protocol for DPP10 antibody 84127-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols