DPP10 Recombinant antibody

DPP10 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241446A5

Cat no : 84127-2-RR

Synonyms

DPL2, Dipeptidyl peptidase-like protein 2, Dipeptidyl peptidase X, Dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase 10

---

Validation Data Gallery

• 

  WB analysis using 84127-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of pig brain using 84127-2-RR

  IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug.

• 

  Affinity and Kinetic Characterization of 84127-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: pig brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84127-2-RR is DPP10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DPP10 fusion protein Ag3231
• Full Name: dipeptidyl-peptidase 10
• Calculated Molecular Weight: 796 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC030832
• Gene Symbol: DPP10
• Gene ID (NCBI): 57628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.