DPEP1 Monoclonal antibody, PBS Only

DPEP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F4

Cat no : 68081-1-PBS

Synonyms

Dehydropeptidase I, Dipeptidase 1, dipeptidase 1 (renal), DPEP1, hRDP, MBD1, MDP, Microsomal dipeptidase, RDP, Renal dipeptidase

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Validation Data Gallery

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  WB analysis of pig pancreas using 68081-1-Ig (same clone as 68081-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 68081-1-Ig (same clone as 68081-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit kidney using 68081-1-Ig (same clone as 68081-1-PBS)

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat pancreas using 68081-1-Ig (same clone as 68081-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68081-1-Ig (same clone as 68081-1-PBS)

  WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells. This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68081-1-Ig (same clone as 68081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68081-1-Ig (same clone as 68081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 68081-1-Ig (same clone as 68081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68081-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68081-1-PBS targets DPEP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DPEP1 fusion protein Ag14591
• Full Name: dipeptidase 1 (renal)
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC017023
• Gene Symbol: DPEP1
• Gene ID (NCBI): 1800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.