DPEP1 Monoclonal antibody

DPEP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B4F4

Cat no : 68081-1-Ig

Synonyms

hRDP, EC:3.5.2.6, EC:3.4.13.19, Dehydropeptidase-I, Beta-lactamase

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Validation Data Gallery

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  WB analysis of pig pancreas using 68081-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 68081-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit kidney using 68081-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 68081-1-Ig

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 68081-1-Ig (DPEP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68081-1-Ig

  WB result of DPEP1 antibody (68081-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPEP1 transfected HepG2 cells.

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  IHC staining of human kidney using 68081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 68081-1-Ig (DPEP1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 68081-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DPEP1 antibody (68081-1-Ig, Clone: 2B4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, rabbit kidney tissue, pig pancreas tissue, rat pancreas tissue, HepG2 cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68081-1-Ig targets DPEP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DPEP1 fusion protein Ag14591
• Full Name: dipeptidase 1 (renal)
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC017023
• Gene Symbol: DPEP1
• Gene ID (NCBI): 1800
• RRID: AB_2918818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.